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minimal s2 media  (Lonza)


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    Structured Review

    Lonza minimal s2 media
    Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila <t>S2</t> cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. <t>B)</t> <t>Aag2</t> cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.
    Minimal S2 Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimal+s2+media/minimal+s2+media/pmc03000392-234-11-13
    Average 90 stars, based on 1 article reviews
    minimal s2 media - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns"

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns

    Journal: BMC Molecular Biology

    doi: 10.1186/1471-2199-11-84

    Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila S2 cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. B) Aag2 cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.
    Figure Legend Snippet: Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila S2 cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. B) Aag2 cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.

    Techniques Used: Construct, Luciferase, Transfection, Control, Plasmid Preparation, Expressing, Lysis, Activity Assay, Virus

    Related Articles

    Construct:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Luciferase:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Transfection:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Control:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Plasmid Preparation:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Expressing:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Lysis:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Activity Assay:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Virus:

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns
    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.



    Similar Products

    minimal s2 media  (Lonza)
    90
    Lonza minimal s2 media
    Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila <t>S2</t> cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. <t>B)</t> <t>Aag2</t> cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.
    Minimal S2 Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimal+s2+media/minimal+s2+media/pmc03000392-234-11-13
    Average 90 stars, based on 1 article reviews
    minimal s2 media - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila S2 cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. B) Aag2 cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.

    Journal: BMC Molecular Biology

    Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns

    doi: 10.1186/1471-2199-11-84

    Figure Lengend Snippet: Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila S2 cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. B) Aag2 cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.

    Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

    Techniques: Construct, Luciferase, Transfection, Control, Plasmid Preparation, Expressing, Lysis, Activity Assay, Virus